Characterization of polyproline-water system by NMR spin grouping and exchange analysis

J. A. Stanley; H. Peemoeller

doi:doi:10.1051/jp2:1991165

Numéro		J. Phys. II France Volume 1, Numéro 12, December 1991


Page(s)		1491 - 1503
DOI		https://doi.org/10.1051/jp2:1991165

DOI: 10.1051/jp2:1991165
J. Phys. II France 1 (1991) 1491-1503

Characterization of polyproline-water system by NMR spin grouping and exchange analysis

J. A. Stanley and H. Peemoeller

Guelph-Waterloo Program for Graduate Work in Physics, Department of Physics, University of Waterloo, Waterloo, Ontario, Canada N2L 3G1

(Received 26 June 1991, accepted 2 September 1991)

Abstract
Proton NMR spin grouping experiments (T₁, T_1p, T_1D) and T₂ measurements were performed in dry and hydrated polyproline powders. The results in the hydrated samples were analyzed for magnetization exchange using a linear three site (proline - ${\rm H_2O}$ - proline) exchange scenario. The intrinsic proline and water proton relaxation parameters as well as the exchange rates were obtained. All proline protons are effectively coupled to the water protons. Perhaps the most significant result is the observation, for the first time, of a very large spin-spin coupling rate $((3.0 \pm 1.5 ) \times 10^3 {\rm s}^{-1})$ between water protons and a small fraction of the polymer proton population.